Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

FGFR4

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal Human Fc and His Tag

Secvență

LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD

Activitate biologică

Immobilized recombinant human FGF2 (1 µg/mL) bind recombinant human FGFR4 in a functional ELISA with a linear range of 30-125 ng/mL. Immobilized recombinant human FGFR4 (5 µg/mL) bind recombinant human FGF12 in a functional ELISA with a linear range of 35-100 ng/mL. Inhibition of the FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts: ED50 = 0.03-0.12 ng/mL.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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