Recombinant Human FGF2 Protein

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg · 100µg

Țintă

FGF2

Formulation

Lyophilized from 20mM Tris, pH 7.5, with 150mM Nacl (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

<1 EU/µg of the protein by LAL method.

Secvență

PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Activitate biologică

Immobilized Human FGF2 (0.5 µg/mL) bind Human GPC3 in a functional ELISA with a linear range of 7-20 ng/mL. Cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts: ED50 = typically 0.635-2.54 ng/mL; Specific activity =3.94 × 10^5~1.57 × 10^6 units/mg. Recombinant Human VEGFA(40 ng/mL) and bFGF (50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells: pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium after 4 days. Primary neural stem cells cultured with bFGF (20 ng/mL): particle size of the suspended neural stem cells gradually increased.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului