Recombinant Human c-Jun Protein (N-terminal His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

2µg · 10µg · 1mg

Țintă

c-Jun

Applications

WB

Formulation

Lyophilized without additives.

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

>95% as determined by SDS-PAGE.

Reconstitution

Centrifuge vial before opening. Reconstitute with 40 mM Tris, pH 7.5, to 0.2-1.0 mg/ml.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Protein Length

Protein fragment

Etichetă (tag)

N-terminal His Tag

Secvență

MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLKLASPELERLII

Activitate biologică

C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

Storage

Shipped at ambient temperature. Short term (1-2 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.

Documentație

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