Recombinant Human BAFF Protein

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

BAFF

Formulation

Lyophilized from 50mM Tris, pH 8.0, with 150mM NaCl and without preservatives or carriers (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 90% (by SDS-PAGE)

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Secvență

AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Activitate biologică

Immobilized recombinant human BAFF (5 µg/mL) bind recombinant human BCMA in a functional ELISA with a linear range of 3-20 ng/mL. Cell proliferation assay using anti-IgM stimulated mouse B cells: ED50 = 0.1-1 ng/mL; Specific activity =1×10^6-1×10^7units/mg. Loaded Human TNFRSF17/BCMA/CD269 Protein, C-Human Fc and His Tag, on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein in BLI assay (Gator) with an affinity constant of 3.05nM.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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