Recombinant Human Adiponectin Protein

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Specificații

Brand

Antibodies

Mărime

25µg

Țintă

Adiponectin

Applications

ELISA, Functional Assays

Formulation

Supplied in 10mM TRIS, pH 8.5 with 75mM L-Arginine.

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Purity

>97% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 25 µl of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Expression system

High-5 insect cells

Nature

Recombinant

Protein species

Human

Endotoxin level

< 1 EU/µg

Specificitate

Recombinant Adiponectin producted in Hi5 cells derived from the cabbage looper moth Trichoplusia ni corresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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