Recombinant Gilthead Seabream IGF1 Protein

Pe scurt

Specificații

Brand

Antibodies

Mărimi disponibile

10µg · 50µg · 1mg

Țintă

IGF1

Applications

Binding Assay

Formulation

Lyophilized from a solution containing 0.02% Sodium Bicarbonate.

Product form

Lyophilized

Concentration

1 mg/ml

Purity

>98% as determined by SEC-HPLC and SDS-PAGE.

Reconstitution

Reconstitute with 0.4% Sodium Bicarbonate adjusted to pH 8-9, to no less than 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Gilthead Seabream

Secvență

MSPETLCGAELVDTLQFVCGERGFYFSKPGYGPNARRSRGIVDECCFQSCELRRLEMYCAPAKTSK

Activitate biologică

Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active.

Storage

Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

Documentație

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