Recombinant Bovine Interferon gamma Protein

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Specificații

Brand

Antibodies

Mărime

25µg

Țintă

Interferon gamma

Applications

ELISA, Functional Assays

Formulation

Supplied in Phosphate Buffered Saline with 10% Trehalose.

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Ion exchange chromatography.

Recommended dilutions

This reagent has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)

Purity

> 95% (by SDS-PAGE).

Reconstitution

Reconstitute with 100 µl of sterile Phosphate Buffered Saline containing 0.1% carrier protein Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Expression system

Pichia pastoris

Nature

Recombinant

Protein species

Bovine

Specificitate

Recombinant Bovine interferon gamma, (IFN?), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.IFN? is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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