Rat Small Intestinal Organoid Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

1 kit

Applications

Cell culture, 3D Cell Culture, Organoids

Reactivity

Rat

Product form

Liquid

Sample type

intestinal

Principiul testului

The organoid culture assay is based on the ability of primary tissue-derived cells to self-organize into three-dimensional structures when provided with an appropriate extracellular matrix and a defined, lineage-specific growth environment. Fresh tissue is first processed using a controlled enzymatic digestion solution that releases viable stem and progenitor cell clusters while preserving surface receptors and cellular polarity. The resulting fragments are embedded in a matrix such as Matrigel, which functions as a scaffold that supports 3D architecture and enables signaling through integrins and basement-membrane interactions. The cultures are then supplied with a specialized organoid medium containing niche-mimicking growth factors, small molecules, and nutrients that maintain stemness while permitting tissue-specific differentiation. During routine expansion, a gentle passaging enzyme is used to fragment mature organoids into smaller units without compromising viability, allowing serial propagation over many passages. For biobanking, a cryopreservation formulation containing permeable and non-permeable cryoprotectants is applied to prevent ice-induced damage and maintain long-term genomic and functional stability. After thawing, a recovery buffer restores isotonic balance and supports rapid re-establishment of 3D growth. Together, this workflow enables the continuous generation, expansion, freezing, and re-culturing of physiologically relevant organoids that retain genetic, phenotypic, and microenvironmental features of the source tissue, making the system suitable for disease modeling, drug screening, and personalized research applications.

Storage

Shipped at 4°C. Store at 4°C with a shelf life of 3 months; store at -20°C with shelf life of 1 year.