Imagine indisponibilăRat Ribonuclease 3/ECP ELISA Kit
Pe scurt
Rat Ribonuclease 3/ECP ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Ribonuclease 3/ECP, cu reactivitate declarată pentru Rat. Măsoară în domeniul 62.5-4000 pg/ml, are o sensibilitate de 28.73 pg/ml, folosește detecție Colourmetric și se poate folosi pe probe de tip Serum, plasma, tissue homogenates or other biological fluids. Se livrează în mărimile 48T și 96T, la prețuri între 4.325,75 și 5.690,03 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat Ribonuclease 3/ECP ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Ribonuclease 3/ECP in serum, plasma, tissue homogenates or other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărimi disponibile
- 48T · 96T
- Țintă
- Ribonuclease 3/ECP
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 28.73 pg/ml
- Detection type
- Colourmetric
- Product range
- 62.5-4000 pg/ml
- Assay Time
- 4h 30m
- Principiul testului
- Rat Ribonuclease 3/ECP ELISA Kit (A82344) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Ribonuclease 3/ECP in serum, plasma, tissue homogenates or other biological fluids. An antibody specific for Ribonuclease 3/ECP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Ribonuclease 3/ECP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Ribonuclease 3/ECP Antibody, which binds the captured Ribonuclease 3/ECP present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Ribonuclease 3/ECP captured in each well. The concentration of Ribonuclease 3/ECP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
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