Imagine indisponibilăRat p38 alpha/MAPK14 ELISA Kit
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Rat p38 alpha/MAPK14 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea p38 alpha/MAPK14, cu reactivitate declarată pentru Rat. Măsoară în domeniul 1.25-80 ng/ml, are o sensibilitate de 0.75 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat p38 alpha/MAPK14 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat p38 alpha/MAPK14 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- p38 alpha/MAPK14
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Recommended dilutions
- Validated sample dilutions include HSC-T6 cell lysate (undiluted) and C6 cell lysate (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.75 ng/ml
- Cross reactivity
- Specific for p38 alpha/MAPK14 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 1.25-80 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Rat p38 alpha/MAPK14 ELISA Kit (A79522) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat p38 alpha/MAPK14 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for p38 alpha/MAPK14. Standards and samples are added to the wells, where p38 alpha/MAPK14 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for p38 alpha/MAPK14 is added to the wells. The detection antibody binds the captured p38 alpha/MAPK14 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of p38 alpha/MAPK14 captured in each well. The concentration of p38 alpha/MAPK14 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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