Rat Melatonin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Melatonin

Applications

Competitive ELISA

Reactivity

Rat

Assay type

Competitive (quantitative)

Sensitivity

9.38 pg/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for Melatonin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15.63 - 1000 pg/ml

Assay Time

2 hours

Principiul testului

Rat Melatonin ELISA Kit (A327102) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Melatonin in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with Melatonin antigen. During the incubation, Melatonin present in the samples or standards competes with the fixed amount of immobilized Melatonin for binding sites on the Biotinylated Anti-Melatonin Antibody. The more Melatonin present in a sample or standard, the less Biotinylated Anti-Melatonin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Melatonin Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Melatonin present in each sample or standard. The concentration of Melatonin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.