Imagine indisponibilăRat Cripto1/CRIPTO ELISA Kit
Pe scurt
Rat Cripto1/CRIPTO ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Cripto1/CRIPTO, cu reactivitate declarată pentru Rat. Măsoară în domeniul 78.125-5000 pg/ml, are o sensibilitate de 46.875 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat Cripto1/CRIPTO ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Cripto1/CRIPTO in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Cripto1/CRIPTO
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 46.875 pg/ml
- Cross reactivity
- Specific for Cripto1/CRIPTO with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 78.125-5000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Rat Cripto1/CRIPTO ELISA Kit (A78854) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Cripto1/CRIPTO in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Cripto1/CRIPTO. Standards and samples are added to the wells, where Cripto1/CRIPTO binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Cripto1/CRIPTO is added to the wells. The detection antibody binds the captured Cripto1/CRIPTO to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Cripto1/CRIPTO captured in each well. The concentration of Cripto1/CRIPTO is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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