Imagine indisponibilăRat Connexin 43/GJA1 ELISA Kit
Pe scurt
Rat Connexin 43/GJA1 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Connexin 43/GJA1, cu reactivitate declarată pentru Rat. Măsoară în domeniul 0.156-10 ng/ml, are o sensibilitate de 0.094 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat Connexin 43/GJA1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Connexin 43/GJA1 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Connexin 43/GJA1
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.094 ng/ml
- Cross reactivity
- Specific for Connexin 43/GJA1 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.156-10 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Rat Connexin 43/GJA1 ELISA Kit (A79250) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Connexin 43/GJA1 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Connexin 43/GJA1. Standards and samples are added to the wells, where Connexin 43/GJA1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Connexin 43/GJA1 is added to the wells. The detection antibody binds the captured Connexin 43/GJA1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Connexin 43/GJA1 captured in each well. The concentration of Connexin 43/GJA1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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