Imagine indisponibilăRat Aromatase ELISA Kit
Pe scurt
Rat Aromatase ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Aromatase, cu reactivitate declarată pentru Rat. Măsoară în domeniul 125-8000 pg/ml, are o sensibilitate de 75 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat Aromatase ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Aromatase in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Aromatase
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Recommended dilutions
- Validated sample dilutions include C6 cell lysate (1:10-1:20) and H9C2 cell lysate (1:20-1:50).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 75 pg/ml
- Cross reactivity
- Specific for Aromatase with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 125-8000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Rat Aromatase ELISA Kit (A76407) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Aromatase in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Aromatase. Standards and samples are added to the wells, where Aromatase binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Aromatase is added to the wells. The detection antibody binds the captured Aromatase to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Aromatase captured in each well. The concentration of Aromatase is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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