Imagine indisponibilăRat alpha 1 Fetoprotein ELISA Kit
Pe scurt
Rat alpha 1 Fetoprotein ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea alpha 1 Fetoprotein, cu reactivitate declarată pentru Rat. Măsoară în domeniul 0.313-20 ng/ml, are o sensibilitate de 0.188 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 3.993,00 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Rat alpha 1 Fetoprotein ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat alpha 1 Fetoprotein in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- alpha 1 Fetoprotein
- Applications
- Sandwich ELISA
- Reactivity
- Rat
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.188 ng/ml
- Cross reactivity
- Specific for alpha 1 Fetoprotein with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.313-20 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Rat alpha 1 Fetoprotein ELISA Kit (A80245) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat alpha 1 Fetoprotein in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for alpha 1 Fetoprotein. Standards and samples are added to the wells, where alpha 1 Fetoprotein binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for alpha 1 Fetoprotein is added to the wells. The detection antibody binds the captured alpha 1 Fetoprotein to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of alpha 1 Fetoprotein captured in each well. The concentration of alpha 1 Fetoprotein is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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