Rabbit TNF alpha ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

Sandwich ELISA

Reactivity

Rabbit

Recommended dilutions

Validated sample dilutions include Healthy serum (undiluted) and Rabbit peripheral blood lymphocyte culture supernatants (1µg/mL LPS stimulation for 12 hours) (undiluted or 1:2).

Assay type

Sandwich (quantitative)

Sensitivity

4.688 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for TNF alpha with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

7.813-500 pg/ml

Assay Time

2 hours

Principiul testului

Rabbit TNF alpha ELISA Kit (A337173) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rabbit TNF alpha in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where TNF alpha binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured TNF alpha. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.