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Porcine Visfatin ELISA Kit
Pe scurt
Porcine Visfatin ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Visfatin, cu reactivitate declarată pentru Porcine. Măsoară în domeniul 0.156-10 ng/ml, are o sensibilitate de 0.094 ng/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 3.560,43 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Porcine Visfatin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine Visfatin in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Visfatin
- Applications
- Sandwich ELISA
- Reactivity
- Porcine
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.094 ng/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for Visfatin with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.156-10 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Porcine Visfatin ELISA Kit (A326961) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine Visfatin in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Visfatin. Standards and samples are added to the wells, where Visfatin binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Visfatin is added to the wells. The detection antibody binds the captured Visfatin to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Visfatin captured in each well. The concentration of Visfatin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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