Imagine indisponibilăPorcine PDGF-AB ELISA Kit
Pe scurt
Porcine PDGF-AB ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea PDGF AB, cu reactivitate declarată pentru Porcine. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.625,23 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Porcine PDGF-AB ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine PDGF-AB in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- PDGF AB
- Applications
- Sandwich ELISA
- Reactivity
- Porcine
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for PDGF AB with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Porcine PDGF-AB ELISA Kit (A335321) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine PDGF-AB in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for PDGF-AB. Standards and samples are added to the wells, where PDGF-AB binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for PDGF-AB is added to the wells. The detection antibody binds the captured PDGF-AB to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of PDGF-AB captured in each well. The concentration of PDGF-AB is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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