Porcine Ghrelin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Ghrelin

Applications

Competitive ELISA

Reactivity

Porcine

Assay type

Competitive (quantitative)

Sensitivity

0.043 ng/ml

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

2h 30m

Principiul testului

Porcine Ghrelin ELISA Kit (A4982) employs the competitive enzyme immunoassay technique for the quantitative measurement of porcine Ghrelin in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The 96-well microtiter plate has been pre-coated with Ghrelin antigen. During the incubation, Ghrelin present in the samples or standards competes with the fixed amount of immobilized Ghrelin for binding sites on the Biotinylated Anti-Ghrelin Antibody. The more Ghrelin present in a sample or standard, the less Biotinylated Anti-Ghrelin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Ghrelin Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Ghrelin present in each sample or standard. The concentration of Ghrelin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului