pH Sensitive IgG Labeling Reagent Plus

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

10µg · 50µg · 100µg

Applications

Antibody Internalization

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Product form

Lyophilized

Concentration

Reconstitution dependent.

Recommended dilutions

We recommend testing the antibody mixture with A331957 at 1:2 molar ratio

Molecular weight

21.8 kDa

Detection type

Flow cytometry, detected with FITC or AF488 filter.

Reconstitution

Reconstitute with distilled sterile water.

Principiul testului

pH Sensitive IgG Labeling Reagent Plus (A331957) offers a simple method to quantify internalization of IgGs of multiple species, exhibiting a lower molecular weight and an enhanced ability to bind to more antibody isotypes than pH Sensitive IgG Labeling Reagent (A331956). After attachment to the Fc region of the antibody, the fluorescent signal of the binding protein indicates internalization by substantially increasing in acidic environments, such as in endosomal and lysosomal vesicles. The strength of fluorescence can therefore be used to measure the efficiency and kinetics of antibody internalization, which are important in assessing antibody-based drug delivery systems and the effectiveness of antibodies in targeted therapeutic or diagnostic applications.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C desiccated and protected from light. Reconstituted: Short term (1-2 weeks) store at 4°C. Long term aliquot and store at -20°C with 50% glycerol. Avoid repeated freeze/thaw cycles.

Documentație

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