NF-kB p65 (phospho Ser536) Cell Based ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

2 x 96T

Țintă

NF-kB p65

Applications

Cell-Based ELISA

Reactivity

Human (Ser536), Mouse (Ser534), Rat (Ser535)

Assay type

Cell-based (qualitative)

Sample type

Adherent and suspension cells.

Cross reactivity

No reported cross-reactivity.

Detection type

Fluorometric

Platform

Tissue Culture Treated Microplate

Product range

> 5000 Cells

Principiul testului

This NF-kB p65 (phospho Ser536) Cell-Based ELISA Kit allows for the detection of NF-kB p65 (phospho Ser536) and the effects that certain stimulation conditions have on NF-kB p65 (phospho Ser536) expression in different cell lines. Qualitative determination of NF-kB p65 (phospho Ser536) concentration is achieved by an indirect ELISA format. In essence, NF-kB p65 (phospho Ser536) is captured by Anti-NF-kB p65 (phospho Ser536) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-NF-kB p65 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Storage

Store at + 4°C. Stable for 3-6 months.