Murine Syndecan 1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Syndecan 1

Applications

Sandwhich ELISA

Reactivity

Murine

Conjugate

Unconjugated

Assay type

Sandwich (quantitative)

Sensitivity

0.35 ng/ml

Sample type

Serum, plasma, and cell culture supernatants.

Cross reactivity

This product shows no cross reactivity with other molecules.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Product range

0.5-16 ng/ml

Assay Time

2h 45m

Specificitate

This product recognizes recombinant and natural murine soluble Syndecan-1.

Principiul testului

Murine Syndecan 1 ELISA Kit (A341771) is a sandwich ELISA designed for the sensitive and quantitative measurement of murine Syndecan 1 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Syndecan 1. Standards and samples are added to the wells, allowing Syndecan 1 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Syndecan 1 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Syndecan 1 in the sample. The concentration of Syndecan 1 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.