Imagine indisponibilăMouse Vimentin ELISA Kit
Pe scurt
Mouse Vimentin ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Vimentin, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 0.313-20 ng/ml, are o sensibilitate de 0.188 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Cell or tissue lysate and other biological fluids. Se livrează în mărimea 96T, la prețul de 3.993,00 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse Vimentin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Vimentin in cell or tissue lysate and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Vimentin
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Recommended dilutions
- Validated sample dilutions include mouse muscle lysate (1:2) and 4T1 cell lysate (1:2).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.188 ng/ml
- Cross reactivity
- Specific for Vimentin with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.313-20 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Mouse Vimentin ELISA Kit (A326962) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Vimentin in cell or tissue lysate and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Vimentin. Standards and samples are added to the wells, where Vimentin binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Vimentin is added to the wells. The detection antibody binds the captured Vimentin to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Vimentin captured in each well. The concentration of Vimentin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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