Mouse TNF alpha ELISA Kit (Small Sample Volume)

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

4.69 pg/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for TNF alpha with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

7.81 - 500 pg/ml

Assay Time

3h 30m

Principiul testului

Mouse TNF alpha ELISA Kit (Small Sample Volume) (A323189) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse in serum, plasma, and other biological fluids. This assay is optimized for limited sample availability, requiring only 25 µL of sample per well. An antibody specific for TNF alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNF alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with biotinylated anti-TNF alpha antibody, which binds the captured TNF alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.