Imagine indisponibilăMouse Tissue Kallikrein ELISA Kit
Pe scurt
Mouse Tissue Kallikrein ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Tissue Kallikrein, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 0.156-10 ng/ml, are o sensibilitate de 0.094 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse Tissue Kallikrein ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Tissue Kallikrein in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Tissue Kallikrein
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.094 ng/ml
- Cross reactivity
- Specific for Tissue Kallikrein with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.156-10 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Mouse Tissue Kallikrein ELISA Kit (A78352) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Tissue Kallikrein in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Tissue Kallikrein. Standards and samples are added to the wells, where Tissue Kallikrein binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Tissue Kallikrein is added to the wells. The detection antibody binds the captured Tissue Kallikrein to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Tissue Kallikrein captured in each well. The concentration of Tissue Kallikrein is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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