Mouse/Rat Insulin like Growth Factor 1 ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Insulin like Growth Factor 1

Applications

Sandwich ELISA

Reactivity

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

0.82 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

15.63 - 1000 pg/ml

Specificitate

This kit can assay both native and recombinant mouse/rat IGF-1.

Principiul testului

Mouse/Rat IGF-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse/rat IGF-1 in the samples. A monoclonal antibody specific for mouse/rat IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IGF-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IGF-1 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage

Store at +4°C. Do not use past expiration date!