Mouse Leptin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Leptin

Applications

Sandwhich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/ml

Sample type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Detection type

Colorimetric

Platform

Microplate

Product range

62.5-4000 pg/ml

Principiul testului

This Leptin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Leptin Antibody. Standards or samples are then added to the microtire plate wells and Leptin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Leptin present in the sample, Anti-Leptin Antibody (Biotin) is added to each well to "sandwich" the Leptin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Leptin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.