Mouse Immunoglobulin Isotyping ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Immunoglobulin Isotyping

Applications

Qualitative ELISA

Reactivity

Mouse

Assay type

Indirect (qualitative)

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Immunoglobulin Isotyping with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

Qualitative

Assay Time

3 hours

Principiul testului

Mouse Immunoglobulin Isotyping ELISA Kit (A246961) is a 3 hour qualitative Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro quantitative determination of mouse Immunoglobulin Isotyping in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Immunoglobulin Isotyping. Standards and samples are added to the wells, where Immunoglobulin Isotyping binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Immunoglobulin Isotyping is added to the wells. The detection antibody binds the captured Immunoglobulin Isotyping to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Immunoglobulin Isotyping captured in each well. The concentration of Immunoglobulin Isotyping is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.