Mouse IgG2c ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IgG2c

Applications

Sandwich ELISA

Reactivity

Mouse

Recommended dilutions

Estimate the expected concentration of samples prior to the assay. For serum and plasma samples, a 2-5 fold dilution is recommended using the sample diluent provided in the kit to maintain assay consistency.

Assay type

Sandwich (quantitative)

Sensitivity

0.19 ng/ml

Sample type

Serum and plasma

Cross reactivity

Specific for IgG2c with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.31-20 ng/ml

Assay Time

1h 30m

Principiul testului

Mouse IgG2c ELISA Kit (A327145) employs a rapid sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of mouse IgG2c in serum and plasma. An antibody specific for IgG2c has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection antibody working solution. During incubation, IgG2c present in the sample binds to the immobilized capture antibody and forms a sandwich immune complex with the HRP-conjugated detection antibody. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IgG2c captured in each well. The concentration of IgG2c is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!