Imagine indisponibilăMouse IGFBP6 ELISA Kit (Small Sample Volume)
Pe scurt
Mouse IGFBP6 ELISA Kit (Small Sample Volume) este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea IGFBP6, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 125-8000 pg/ml, are o sensibilitate de 75.22 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma and other biological fluids. Se livrează în mărimea 96T, la prețul de 3.893,18 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse IGFBP6 ELISA Kit (Small Sample Volume) is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IGFBP6 in serum, plasma, and other biological fluids.. This ELISA kit only requires 25µl of sample volume(s).
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- IGFBP6
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 75.22 pg/ml
- Sample type
- Serum, plasma and other biological fluids.
- Cross reactivity
- Specific for IGFBP6 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 125-8000 pg/ml
- Assay Time
- 3h 30m
- Principiul testului
- Mouse IGFBP6 ELISA Kit (Small Sample Volume) (A323218) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse in serum, plasma, and other biological fluids. This assay is optimized for limited sample availability, requiring only 25 µL of sample per well. An antibody specific for IGFBP6 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IGFBP6 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with biotinylated anti-IGFBP6 antibody, which binds the captured IGFBP6 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IGFBP6 captured in each well. The concentration of IGFBP6 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
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