Mouse GAPDH ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

GAPDH

Applications

Sandwich ELISA

Reactivity

Mouse

Recommended dilutions

Validated sample dilutions include 4T1 cell lysate (1:1000-1:2000) and Raw264.7 cell lysate (1:1000-1:2000).

Assay type

Sandwich (quantitative)

Sensitivity

0.188 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for GAPDH with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.313-20 ng/ml

Assay Time

4 hours

Principiul testului

Mouse GAPDH ELISA Kit (A76595) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GAPDH in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for GAPDH. Standards and samples are added to the wells, where GAPDH binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for GAPDH is added to the wells. The detection antibody binds the captured GAPDH to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of GAPDH captured in each well. The concentration of GAPDH is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului