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Mouse Galectin 2 ELISA Kit
Pe scurt
Mouse Galectin 2 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Galectin 2, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.186,00 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse Galectin 2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Galectin 2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Galectin 2
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Recommended dilutions
- Validated sample dilutions include Healthy serum (1:25-1:50) and Healthy plasma (1:25-1:50).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for Galectin 2 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Mouse Galectin 2 ELISA Kit (A336542) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Galectin 2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where Galectin 2 binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured Galectin 2. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Galectin 2 captured in each well. The concentration of Galectin 2 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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