Mouse GAD65 IgM Autoantibodies ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

GAD65 IgM Autoantibodies

Applications

Indirect ELISA

Reactivity

Mouse

Assay type

Indirect (quantitative)

Sensitivity

0.469 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for GAD65 IgM Autoantibodies with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.781-50 ng/ml

Assay Time

4 hours

Principiul testului

Mouse GAD65 IgM Autoantibodies ELISA Kit (A73997) is a 4 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of mouse GAD65 IgM Autoantibodies in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where GAD65 IgM Autoantibodies binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured GAD65 IgM Autoantibodies to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of GAD65 IgM Autoantibodies present in the sample. The concentration of GAD65 IgM Autoantibodies is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.