Imagine indisponibilăMouse Cytochrome b5 ELISA Kit
Pe scurt
Mouse Cytochrome b5 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Cytochrome b5, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse Cytochrome b5 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Cytochrome b5 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Cytochrome b5
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Cross reactivity
- Specific for Cytochrome b5 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Mouse Cytochrome b5 ELISA Kit (A77555) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Cytochrome b5 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Cytochrome b5. Standards and samples are added to the wells, where Cytochrome b5 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Cytochrome b5 is added to the wells. The detection antibody binds the captured Cytochrome b5 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Cytochrome b5 captured in each well. The concentration of Cytochrome b5 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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