Imagine indisponibilăMouse Cyclophilin B ELISA Kit
Pe scurt
Mouse Cyclophilin B ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Cyclophilin B, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 125-8000 pg/ml, are o sensibilitate de 75 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse Cyclophilin B ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Cyclophilin B in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Cyclophilin B
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 75 pg/ml
- Cross reactivity
- Specific for Cyclophilin B with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 125-8000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Mouse Cyclophilin B ELISA Kit (A76418) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Cyclophilin B in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Cyclophilin B. Standards and samples are added to the wells, where Cyclophilin B binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Cyclophilin B is added to the wells. The detection antibody binds the captured Cyclophilin B to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Cyclophilin B captured in each well. The concentration of Cyclophilin B is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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