Mouse CXCL1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CXCL1

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

0.47 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

15.63 - 1000 pg/ml

Recovery

98% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant mouse CXCL1.

Principiul testului

Mouse CXCL1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CXCL1 in the samples. A monoclonal antibody specific for mouse CXCL1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and CXCL1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for CXCL1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!