Mouse Anti-KLHIgM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Anti-KLH IgM

Applications

Capture ELISA

Reactivity

Mouse

Assay type

Capture (quantitative)

Sensitivity

0.938 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for Anti-KLH IgM with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.563-100 ng/ml

Assay Time

4 hours

Principiul testului

Mouse Anti-KLH IgM ELISA Kit (High Range) (A326376) is a capture Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Anti-KLH IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with a capture antibody specific for Anti-KLH IgM. Standards and samples are added to the wells, where Anti-KLH IgM present in each sample binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an assay-specific detection reagent is added to the wells. This detection reagent binds the captured Anti-KLH IgM to form a capture immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-KLH IgM captured in each well. The concentration of Anti-KLH IgM is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.