Mouse anti-Human EPO IgM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

anti-Human EPO IgM

Applications

Indirect ELISA

Reactivity

Mouse

Assay type

Capture (quantitative)

Sensitivity

0.938 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for anti-Human EPO IgM with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.563-100 ng/ml

Assay Time

3 hours

Principiul testului

Mouse anti-Human EPO IgM ELISA Kit (A335216) is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of mouse anti-Human EPO IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where anti-Human EPO IgM binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured anti-Human EPO IgM to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of anti-Human EPO IgM present in the sample. The concentration of anti-Human EPO IgM is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.