Imagine indisponibilăMouse 4-1BBL ELISA Kit
Pe scurt
Mouse 4-1BBL ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea 4-1BBL, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 78.125-5000 pg/ml, are o sensibilitate de 46.875 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.186,00 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse 4-1BBL ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse 4-1BBL in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- 4-1BBL
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Recommended dilutions
- Validated sample dilutions include Healthy serum (Undiluted) and Mouse peripheral blood Cell Culture Supernatants (1µg/mL LPS stimulation for 12 hours) (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 46.875 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for 4-1BBL with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 78.125-5000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Mouse 4-1BBL ELISA Kit (A336651) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse 4-1BBL in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where 4-1BBL binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured 4-1BBL. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of 4-1BBL captured in each well. The concentration of 4-1BBL is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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