Monkey Firefly Luciferase IgM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Firefly Luciferase IgM

Applications

Capture ELISA

Reactivity

Monkey

Assay type

Capture (quantitative)

Sensitivity

0.469 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for Firefly Luciferase IgM with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.781-50 ng/ml

Assay Time

3 hours

Cas number

CAS:61970-00-1

Principiul testului

Monkey Firefly Luciferase IgM ELISA Kit (A335286) is a capture Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of monkey Firefly Luciferase IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with a capture antibody specific for Firefly Luciferase IgM. Standards and samples are added to the wells, where Firefly Luciferase IgM present in each sample binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an assay-specific detection reagent is added to the wells. This detection reagent binds the captured Firefly Luciferase IgM to form a capture immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Firefly Luciferase IgM captured in each well. The concentration of Firefly Luciferase IgM is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.