Imagine indisponibilăMonkey Factor X ELISA Kit
Pe scurt
Monkey Factor X ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Factor X, cu reactivitate declarată pentru Monkey. Măsoară în domeniul 0.625-40 ng/ml, are o sensibilitate de 0.375 ng/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.625,23 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Monkey Factor X ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey Factor X in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Factor X
- Applications
- Sandwich ELISA
- Reactivity
- Monkey
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.375 ng/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for Factor X with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.625-40 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Monkey Factor X ELISA Kit (A335250) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey Factor X in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Factor X. Standards and samples are added to the wells, where Factor X binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Factor X is added to the wells. The detection antibody binds the captured Factor X to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Factor X captured in each well. The concentration of Factor X is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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