mIHC Lung Cancer TME Panel I - 5 Color

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Descriere

Specificații

Brand

Antibodies

Mărime

20T

Țintă

PD-L1 + PD 1 + Ki67 + SOX2 + GSTP1

Applications

IHC, mIHC, TMA

Reactivity

Primary antibody: Human; Secondary antibody: Rabbit and Mouse

Conjugate

Secondary antibody: HRP

Host

Primary antibody: Rabbit; secondary antibody: Goat

Product form

Liquid

Recommended dilutions

The fluorescent dye is provided as a 100× stock solution. To prepare the working solution, dilute the stock 1:100 with the freshly prepared signal amplification reaction solution. DAPI should be diluted 1:100 in sterile water to obtain its working solution. The kit also contains a universal secondary antibody compatible with mouse and rabbit primary antibodies; before use, verify that the primary antibody species matches the secondary antibody. The secondary antibody is supplied as a 1× working solution and is ready for use.

Sample type

Lung cancer.

Emission maxima

520 nm, 570 nm, 620 nm, 650 nm, 700 nm

Principiul testului

This multiplex fluorescence immunohistochemical assay is based on the specific antigen-antibody interaction combined with tyramide signal amplification (TSA) technology. Similar to the DAB chromogenic reaction used in conventional IHC, TSA employs horseradish peroxidase (HRP)-conjugated secondary antibodies to catalyze the activation of a tyramide-linked fluorophore substrate. The activated fluorophore covalently binds to tyrosine residues in proximity to the target antigen, generating a stable and permanent fluorescent signal. Following each staining cycle, non-covalently bound antibodies are removed through heat-mediated antigen retrieval, enabling subsequent rounds of staining with different primary antibodies and fluorophores. Through iterative cycles, multiple biomarkers, such as PD-L1, PD 1, Ki67, SOX2, and GSTP1, can be sequentially labeled within the same lung cancer tissue section. This approach allows high-resolution visualization of spatial co-expression patterns and cellular interactions within the tumor microenvironment, providing a comprehensive molecular and cellular profile critical for diagnostic and translational research.

Storage

Shipped at +4 °C. Fluorescent dyes should be stored protected from light at +4 °C and are stable for up to one year from the date of receipt.