mIHC Complete TSA Multiplex Kit - 6 Color

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Specificații

Brand

Antibodies

Mărimi disponibile

20T · 100T · 50T

Applications

IHC, mIHC, TMA

Reactivity

Rabbit

Conjugate

HRP

Host

Goat

Product form

Liquid

Recommended dilutions

The fluorescent dye is provided as a 100× stock solution. The working solution is prepared by diluting the stock 1:100 in the freshly prepared signal amplification reaction solution. DAPI should be diluted 1:100 in sterile water to prepare its working solution. The kit also contains a secondary antibody specific for rabbit primary antibodies; before use, confirm that the primary antibody species matches the secondary antibody. The secondary antibody is supplied as a 1× working solution and is ready for use.

Sample type

This kit is suitable for paraffin-embedded tissues, frozen tissues, tissue microarrays (TMA), and cell climbing slides.

Emission maxima

520 nm, 570 nm, 620 nm, 690 nm, 770 nm

Principiul testului

The multiplex fluorescence immunohistochemical assay is based on the specific binding between antigens and antibodies, combined with the principle of tyramide signal amplification (TSA). Similar to the DAB chromogenic reaction in conventional IHC, TSA technology utilizes horseradish peroxidase (HRP)-conjugated secondary antibodies. HRP catalyzes the conversion of a tyramide-linked fluorophore substrate into an activated intermediate that covalently binds to tyrosine residues within the antigen site, producing a stable and permanent fluorescent signal. After each labeling cycle, non-covalently bound antibodies are removed through heat-mediated antigen retrieval, allowing the application of a new primary antibody and a different fluorophore substrate in subsequent staining rounds. By repeating this process, multiple targets can be sequentially labeled within a single tissue section, enabling in situ multiplex detection and visualization of the complex cellular architecture of the tissue microenvironment with high spatial resolution.

Storage

Shipped at +4 °C. Fluorescent dyes should be stored protected from light at +4 °C and are stable for up to one year from the date of receipt.