Imagine indisponibilăHuman TRAIL R4 ELISA Kit
Pe scurt
Human TRAIL R4 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea TRAIL R4, cu reactivitate declarată pentru Human. Măsoară în domeniul 312.5-10000 pg/ml, are o sensibilitate de 64 pg/ml, folosește detecție Colorimetric și se poate folosi pe probe de tip Serum, plasma, and cell culture supernatants. Se livrează în mărimea 96T, la prețul de 6.155,88 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human TRAIL R4 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human TRAIL R4 in biological samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- TRAIL R4
- Applications
- Sandwhich ELISA
- Reactivity
- Human
- Conjugate
- Unconjugated
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 64 pg/ml
- Cross reactivity
- This product shows no cross reactivity was observed with TRAIL, CD117, Il-6R, IL-2R, CD116, TRAIL R1, TRAIL R2, TRAIL R3, CD178 and Granzyme B.
- Detection type
- Colorimetric
- Platform
- Pre-coated Microplate (1 x 96)
- Product range
- 312.5-10000 pg/ml
- Assay Time
- 3h 45m
- Specificitate
- Specific for human TRAIL R4 / DcR2 / TRUNDD.
- Principiul testului
- Human TRAIL R4 ELISA Kit (A341749) is a sandwich ELISA designed for the sensitive and quantitative measurement of human TRAIL R4 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for TRAIL R4. Standards and samples are added to the wells, allowing TRAIL R4 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for TRAIL R4 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of TRAIL R4 in the sample. The concentration of TRAIL R4 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
- Storage
- Store at 4°C.
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