Imagine indisponibilăHuman TNF beta ELISA Kit
Pe scurt
Human TNF beta ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea TNF beta, cu reactivitate declarată pentru Human. Măsoară în domeniul 15.63 - 1000 pg/ml, are o sensibilitate de 2.02 pg/ml, folosește detecție Colorimetric și se poate folosi pe probe de tip Cell culture supernatant, serum and plasma. Se livrează în mărimea 96T, la prețul de 3.726,80 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human TNF beta ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TNF beta in cell culture supernatant, serum and plasma.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- TNF beta
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 2.02 pg/ml
- Sample type
- Cell culture supernatant, serum and plasma.
- Cross reactivity
- This ELISA kit has shown no significant cross-reactivity.
- Detection type
- Colorimetric
- Platform
- Microplate
- Product range
- 15.63 - 1000 pg/ml
- Specificitate
- This kit can assay both native and recombinant human TNF-ß.
- Principiul testului
- Human TNF-ß ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TNF-ß in the samples. A monoclonal antibody specific for human TNF-ß has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TNF-ß, and TNF-ß present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-ß bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
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