Human TNF alpha ELISpot Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

ELISpot

Reactivity

Human

Host

Human

Assay type

sandwich ELISA

Sample type

Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.

Detection type

Colorimetric

Platform

Precoated PVDF Microplate

Assay Time

Assay time: 3 hours. Sample incubation time: 16-24 hours.

Specificitate

This ELISpot Kit specifically targets Human TNF alpha.

Principiul testului

The Human TNF alpha ELISpot Kit (A334659) employs the double-antibody sandwich ELISpot technique for the detection of TNF alpha-secreting cells. A capture antibody specific for TNF alpha has been pre-coated onto a 96-well microplate. Test cells, such as PBMCs, are added into the wells, and upon stimulation with a cell stimulant, TNF alpha secreted by individual cells is captured by the immobilized antibody. A biotinylated anti-TNF alpha detection antibody, which also binds the captured cytokine, and Streptavidin-HRP, which binds the biotinylated detection antibody, are then added, and the plate is incubated. Following incubation, unbound detection antibody and Streptavidin-HRP are removed by washing, and AEC substrate solution is added. Colored spots develop at the sites of cytokine secretion in proportion to the number of TNF alpha-producing cells. The intensity and number of spots can then be quantified using an ELISpot reader. This ready-to-use kit includes pre-coated plates, detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, and washing buffer. For research use only. Not for diagnostic purposes.

Storage

Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului