Human TNF alpha ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and Human peripheral blood leukocyte cells (3 x 10^6 cells/mL) Cell Culture Supernatant (48h) , Stimulated by LPS(10µg/mL) (1:5-1:10).

Assay type

Sandwich (quantitative)

Sensitivity

1.875 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for TNF alpha with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

3.125-200 pg/ml

Assay Time

4 hours

Principiul testului

Human TNF alpha ELISA Kit (High Sensitivity) (A326927) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TNF alpha in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for TNF alpha. Standards and samples are added to the wells, where TNF alpha binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for TNF alpha is added to the wells. The detection antibody binds the captured TNF alpha to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului