Human Thyroxine/T4 Antibody ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Thyroxine/T4 Antibody

Applications

Indirect ELISA

Reactivity

Human

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and Healthy plasma (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

28.125 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Demonstrates specificity for Thyroxine/T4 antibodies with no reported cross-reactivity to related analogs.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

46.875-3000 ng/ml

Assay Time

3 hours

Principiul testului

Human Thyroxine/T4 Antibody ELISA Kit (A78835) is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Thyroxine/T4 Antibody in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Thyroxine/T4 Antibody binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured Thyroxine/T4 Antibody to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Thyroxine/T4 Antibody present in the sample. The concentration of Thyroxine/T4 Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.