Imagine indisponibilăHuman TGF beta 3 ELISA Kit
Pe scurt
Human TGF beta 3 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea TGF beta 3, cu reactivitate declarată pentru Human. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 3.993,00 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human TGF beta 3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TGF beta 3 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- TGF beta 3
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include Healthy serum (1:2) and Hela cell lysate (1:2).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Cross reactivity
- Specific for TGF beta 3 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Human TGF beta 3 ELISA Kit (A77414) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TGF beta 3 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for TGF beta 3. Standards and samples are added to the wells, where TGF beta 3 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for TGF beta 3 is added to the wells. The detection antibody binds the captured TGF beta 3 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TGF beta 3 captured in each well. The concentration of TGF beta 3 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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