Human Semaphorin 3F ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Semaphorin 3F

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

19.25 pg/ml

Sample type

Serum, plasma, tissue homogenates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

46.8-3000 pg/ml

Assay Time

4h 30m

Principiul testului

Human Semaphorin 3F ELISA Kit (A5654) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Semaphorin 3F in serum, plasma, tissue homogenates or other biological fluids. An antibody specific for Semaphorin 3F has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Semaphorin 3F present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Semaphorin 3F Antibody, which binds the captured Semaphorin 3F present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Semaphorin 3F captured in each well. The concentration of Semaphorin 3F can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului